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Plant transformation and editing technologies
Table of contents
Agrobacterium and T-DNA
- T-DNA-genome Junctions Form Early After Infection and Are Influenced by the Chromatin State of the Host Genome
- Transcriptomic Analysis of Arabidopsis Seedlings in Response to an Agrobacterium-Mediated Transformation Process
- Suppression of different classes of somatic mutations in Arabidopsis by vir gene-expressing Agrobacterium strains
- Overexpression of Several Arabidopsis Histone Genes Increases Agrobacterium-Mediated Transformation and Transgene Expression in Plants
- Membrane and Core Periplasmic Agrobacterium Tumefaciens Virulence Type IV Secretion System Components Localize to Multiple Sites Around the Bacterial Perimeter During Lateral Attachment to Plant Cells
- An Improved Recombineering Toolset for Plants [ "BACs" ]
Transgene silencing
- Invasive DNA elements modify the nuclear architecture of their insertion site by KNOT-linked silencing in Arabidopsis thaliana
- Evolution of sequence-specific anti-silencing systems in Arabidopsis
Transformation
- Use of non-integrating Zm-Wus2 vectors to enhance maize transformation
- An innovative automated active compound screening system allows high-throughput optimization of somatic embryogenesis in Coffea arabica
- An efficient method for Agrobacterium-mediated genetic transformation of chilli pepper (Capsicum annuum L.)
- Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor
- Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies
- Genome Editing in Cotton with the CRISPR/Cas9 System
- Arabidopsis NahG Plants as a Suitable and Efficient System for Transient Expression using Agrobacterium tumefaciens
Transformation technology
- Carbon nanocarriers deliver siRNA to intact plant cells for efficient gene knockdown
Viral delivery
- Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
- A barley stripe mosaic virus‐based guide RNA delivery system for targeted mutagenesis in wheat and maize
- A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation in Arabidopsis thaliana
- Efficient Cas9 multiplex editing using unspaced gRNA arrays engineering in a Potato virus X vector
Prime editing in plants
- Prime editing efficiently generates W542L and S621I double mutations in two ALS genes of maize
Technologies
- Recombinase-mediated integration of a multigene cassette in rice leads to stable expression and inheritance of the stacked locus
- Development of mPing-based Activation Tags for Crop Insertional Mutagenesis
CRISPR
- Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9 ["genomic safe harbors"]
Screening
- High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize